| SSC North America | West Richland | 1999 |
| Staples Inc. | Framingham | 1986 |
| Starwood | White Plains | 1930 |
| State Street Corporation | Boston | 1792 |
| Steak 'n Shake | Indianapolis | 1934 |
| Strategic Simulations | Sunnyvale | 1979 |
| Stratolaunch Systems | Montgomery, Seattle | 2011 |
| Stüssy, Inc. | Irvine | 1980 |
| Substack | — | November 29, 2017 |
| Sunkist Growers | Los Angeles | August 29, 1893 |
| Sunoco | Dallas | 1886 |
| Swift Engineering | San Clemente | 1983 |
| Synaptics | Santa Clara | 1986 |
| Synopsys | Mountain View | 1986 |
| T. Rowe Price | Baltimore | 1937 |
| Telltale Games | San Rafael | July 2004 |
| The Bank of New York Mellon | New York City | 2007 |
| The Cigna Group | Bloomfield | 1982 |
| The Gillette Company | — | September 28, 1901 |
| The Travelers Companies | New York City | 1853 |
| The Trump Organization | Trump Tower | 1923 |
| Thinking Machines Corporation | Carter's Ink Building | 1983 |
| TiVo Corporation | Santa Clara, San Jose | 1983 |
| TJX Companies | Framingham | 1956 |
| TopCoder | San Francisco | 2001 |
| Trident Microsystems | Santa Clara | 1987 |
| Trimble | Westminster | 1978 |
| Tripadvisor | Needham | February 14, 2000 |
| TRW Inc. | Euclid | 1901 |
| Turner Broadcasting System | CNN Center | 1970 |
| Tyson Foods | Springdale | 1935 |
| U.S. Steel | Pittsburgh | 2001 |
| Ultimate Fighting Championship | Nevada, Las Vegas | 1993 |
| Union Carbide | Danbury | 1957 |
| United Airlines Holdings | Chicago | December 30, 1968 |
| United Press International | Washington, D.C. | 1907 |
| United Space Alliance | Houston | August 1995 |
| United Technologies Corporation | Hartford | 1929 |
| UnitedHealth Group | Minnetonka | 1977 |
| USA Today Co. | Tysons, New York City | October 6, 1906 |
| Valero Energy | — | 1980 |
| Verbatim | Charlotte | April 28, 1969 |
| Vertiv | Westerville | 1965 |
| Visa Inc. | Mission Rock | 1958 |
| Walmart | Bentonville | October 31, 1969 |
| Warner Bros. Discovery | New York City | April 8, 2022 |
| WarnerMedia | Delaware, New York City | 2016 |
| Warrior Sports | Warren | 1992 |