| Epiphone | Nashville | 1873 |
| Epyx | San Francisco | 1978 |
| Equifax | Atlanta | 1899 |
| ESPN+ | United States | April 12, 2018 |
| Estée Lauder Companies | Neuilly-sur-Seine, New York City | 1946 |
| Ethyl Corporation | Richmond | 1921 |
| Eurasia Group | New York City | 1998 |
| Everlast | New York City | 1910 |
| Exelon | Chicago | 2000 |
| Extreme Championship Wrestling | Philadelphia | April 25, 1992 |
| Exxon | Las Colinas | January 1, 1973 |
| Eye-Fi | Mountain View | 2005 |
| Fairmont Hotels & Resorts | Toronto | 1907 |
| Fandango | Los Angeles | 2000 |
| Fantasy Flight Games | Roseville | 1995 |
| Farmers Insurance Group | Wilshire Boulevard, Los Angeles | 1928 |
| Fastenal | Winona | 1967 |
| Feastables | Chicago | April 2021 |
| FedEx Office | Dallas | 1970 |
| FEI Company | Hillsboro | 1971 |
| Fender Musical Instruments Corporation | Nabari Station | 1946 |
| Fidelity National Information Services | — | February 1, 2006 |
| Firehouse Subs | — | 1994 |
| First Data | Atlanta | 1969 |
| First Solar | Tempe | 1999 |
| Fiserv | Brookfield | 1984 |
| Fisher-Price | New York | June 19, 1930 |
| Florsheim Shoes | Glendale | 1892 |
| Fluor Corporation | Irving | 1890 |
| FMC Corporation | BNY Mellon Center | 1883 |
| FMC Technologies | Houston | 2000 |
| Foot Locker | New York City | 1974 |
| Ford Aerospace | Milpitas | 1956 |
| Forrester | Cambridge | 1983 |
| Fortinet | Sunnyvale | 2000 |
| Fox Television Stations | New York City | 1986 |
| Frigidaire | Charlotte | 1918 |
| Frontier Airlines | Denver | 1994 |
| Fry's Electronics | San Jose | 1985 |
| Fusion-io | Cottonwood Heights | 2006 |
| Galoob | South San Francisco | 1957 |
| Gap Inc. | San Francisco | 1969 |
| GE Aerospace | Evendale | April 15, 1892 |
| GE HealthCare | Chicago | 2004 |
| GE Transportation | Chicago, Heller International Building | 1907 |
| General Atomics | San Diego | 1955 |
| Genesys | Daly City | 1990 |
| Gensler | San Francisco | 1965 |