| E-mu Systems | business, musical instrument manufacturing company | Scotts Valley | 1971 |
| E-Trade | business, enterprise, public company | New York City | 1982 |
| E-ZPass | business | Wilmington | 1987 |
| EA Orlando | video game developer | Orlando | 1994 |
| EA Sports | business | Burnaby | 1991 |
| Eagle | car brand, automobile manufacturer | — | 1987 |
| Eastern Illinois University | public educational institution of the United States, school of pedagogy, public university | — | 1895 |
| Eastern Oregon University | public educational institution of the United States, public university | — | 1929 |
| Eastern Washington University | public educational institution of the United States, university | — | 1882 |
| Eastman School of Music | conservatory | — | 1921 |
| Eaton Corporation | business, enterprise, public company | Cleveland | 1911 |
| EBSCO Information Services | business | Birmingham | 1943 |
| Eckert–Mauchly Computer Corporation | business | Philadelphia | 1948 |
| Eckō Unltd. | business, subsidiary company, brick and mortar | East Brunswick | 1993 |
| Econometric Society | open-access publisher, scientific society, learned society | — | December 29, 1930 |
| Edelman | public relations agency, consulting company, business | New York City | 1952 |
| Edison Manufacturing Company | film production company, business | New York | 1889 |
| Edison Studios | film production company, film studio | United States | 1882 |
| Edmodo | skill, website, business | San Mateo | September 1, 2008 |
| Einstein Bros. Bagels | restaurant chain, business, public company | Lakewood | 1995 |
| El Capitan Theatre | movie theater, movie palace | — | 1926 |
| Elegant Angel | pornographic film studio | Los Angeles | 1990 |
| Elon University | university | — | 1889 |
| Embry–Riddle Aeronautical University | private not-for-profit educational institution, university, open-access publisher | Daytona Beach | 1926 |
| Emerson Electric | business, enterprise, public company | St. Louis | September 24, 1890 |
| EMG, Inc. | business, musical instrument manufacturing company | Santa Rosa | 1976 |
| Encyclopædia Britannica Inc. | book publisher, business | Chicago | 1768 |
| Endeavor Air | regional airline, airline | Fort Snelling | 1985 |
| Energizer Holdings, Inc. | brand, business, enterprise, public company | St. Louis | 2000 |
| Ensoniq | business, musical instrument manufacturing company, enterprise | Malvern | 1982 |
| Enterprise Products | business, public company | Houston | 1968 |
| EnterpriseDB | business | Bedford | March 2004 |
| Envoy Air | airline | Fort Worth | 1984 |
| Epiphone | brand, business | Nashville | 1873 |
| Epyx | video game publisher, video game developer, business, enterprise | San Francisco | 1978 |
| Equifax | credit bureau, business, public company | Atlanta | 1899 |
| Esalen Institute | retreat center, 501(c)(3) organization, educational institution | Slates Hot Springs | 1962 |
| ESPN+ | brand, business, Internet television | United States | April 12, 2018 |
| Essanay Studios | film production company, film studio | Chicago | 1907 |
| Esso | brand, gas station chain | Irving | 1911 |
| Estée Lauder Companies | business, enterprise, public company | Neuilly-sur-Seine, New York City | 1946 |
| Ethyl Corporation | organization, business, public company | Richmond | 1921 |
| Eurasia Group | consulting company, business | New York City | 1998 |
| Evergreen International Airlines | airline | McMinnville | 1960 |
| Evergreen State College | liberal arts college, Colleges That Change Lives, public educational institution of the United States | — | 1967 |
| Everlast | business, enterprise | New York City | 1910 |
| Exelon | holding company, business, enterprise, public company | Chicago | 2000 |
| Expedia.com | online travel agency, travel website, travel agency, mobile app | — | October 22, 1996 |
| ExpressJet | airline | Atlanta | 1986 |
| Extreme Championship Wrestling | media franchise, business | Philadelphia | April 25, 1992 |
| Exxon | oil company, business, gas station chain, public company | Las Colinas | January 1, 1973 |
| Eye-Fi | business, company | Mountain View | 2005 |