| Embry–Riddle Aeronautical University | private not-for-profit educational institution, university, open-access publisher | Daytona Beach | 1926 |
| Emerson Electric | business, enterprise, public company | St. Louis | September 24, 1890 |
| EMG, Inc. | business, musical instrument manufacturing company | Santa Rosa | 1976 |
| Encyclopædia Britannica Inc. | book publisher, business | Chicago | 1768 |
| Endeavor Air | regional airline, airline | Fort Snelling | 1985 |
| Energizer Holdings, Inc. | brand, business, enterprise, public company | St. Louis | 2000 |
| Ensoniq | business, musical instrument manufacturing company, enterprise | Malvern | 1982 |
| Enterprise Products | business, public company | Houston | 1968 |
| EnterpriseDB | business | Bedford | March 2004 |
| Envoy Air | airline | Fort Worth | 1984 |
| Epiphone | brand, business | Nashville | 1873 |
| Epyx | video game publisher, video game developer, business, enterprise | San Francisco | 1978 |
| Equifax | credit bureau, business, public company | Atlanta | 1899 |
| Esalen Institute | retreat center, 501(c)(3) organization, educational institution | Slates Hot Springs | 1962 |
| ESPN+ | brand, business, Internet television | United States | April 12, 2018 |
| Essanay Studios | film production company, film studio | Chicago | 1907 |
| Esso | brand, gas station chain | Irving | 1911 |
| Estée Lauder Companies | business, enterprise, public company | Neuilly-sur-Seine, New York City | 1946 |
| Ethyl Corporation | organization, business, public company | Richmond | 1921 |
| Eurasia Group | consulting company, business | New York City | 1998 |
| Evergreen International Airlines | airline | McMinnville | 1960 |
| Evergreen State College | liberal arts college, Colleges That Change Lives, public educational institution of the United States | — | 1967 |
| Everlast | business, enterprise | New York City | 1910 |
| Exelon | holding company, business, enterprise, public company | Chicago | 2000 |
| Expedia.com | online travel agency, travel website, travel agency, mobile app | — | October 22, 1996 |
| ExpressJet | airline | Atlanta | 1986 |
| Extreme Championship Wrestling | media franchise, business | Philadelphia | April 25, 1992 |
| Exxon | oil company, business, gas station chain, public company | Las Colinas | January 1, 1973 |
| Eye-Fi | business, company | Mountain View | 2005 |
| F5, Inc. | software company, public company | Seattle | 1996 |
| Fairchild | aerospace manufacturer | — | 1925 |
| Fairfax County Public Schools | school division of Virginia | — | 1870 |
| Fairleigh Dickinson University | nonprofit organization, private not-for-profit educational institution, university | — | 1942 |
| Fairmont Hotels & Resorts | hotel brand, hotel chain, business | Toronto | 1907 |
| Fandango | online film database, website, business | Los Angeles | 2000 |
| Fantagraphics Books | comics publishing company, book publisher | Seattle | 1976 |
| Fantasy Flight Games | tabletop role-playing game publisher, board game publishing company, business | Roseville | 1995 |
| Farmers Insurance Group | insurance company, business | Wilshire Boulevard, Los Angeles | 1928 |
| Farrar, Straus and Giroux | publishing house | New York City | 1946 |
| Fastenal | business, enterprise, public company | Winona | 1967 |
| Fatburger | restaurant chain | Santa Monica, Los Angeles | 1952 |
| Feastables | business, company | Chicago | April 2021 |
| Federal Reserve Bank of Atlanta | organization, Federal Reserve Bank | Atlanta | May 18, 1914 |
| Federal Reserve Bank of Cleveland | organization, Federal Reserve Bank | Federal Reserve Bank Building | May 18, 1914 |
| Federal Reserve Bank of Dallas | organization, Federal Reserve Bank | Dallas | 1914 |
| Federal Reserve Bank of Minneapolis | publishing house, organization, Federal Reserve Bank | Minneapolis | 1914 |
| Federal Reserve Bank of New York | Federal Reserve Bank | New York City | 1914 |
| FedEx Office | business, retail chain | Dallas | 1970 |