| Bates College | liberal arts college, private not-for-profit educational institution | — | 1855 |
| Bausch & Lomb | organization, business, enterprise, public company | Vaughan, Rochester | 1853 |
| Baylor University | church college, private not-for-profit educational institution, private university | — | 1845 |
| BBDO | advertising agency | New York City | 1891 |
| Be Inc. | business | Menlo Park | 1990 |
| BEA Systems | business, public company | San Jose | 1995 |
| Beacon Theatre | theatre building, movie theater | — | December 24, 1929 |
| Beatport | online music store, business | — | 2004 |
| Bechtel | organization, business, enterprise | Reston | 1906 |
| Bed Bath & Beyond Inc. | former entity, retail chain, public company | Wilmington | 1971 |
| Bell & Howell | production company, business | Wheeling | 1907 |
| Bell Labs | privately held company, publishing house, research institute | Murray Hill | 1925 |
| Bellagio Hotel & Casino | skyscraper, casino, hotel | — | 1998 |
| Beloit College | liberal arts college, private not-for-profit educational institution | — | 1846 |
| Benchmark | business, enterprise | Menlo Park | 1995 |
| Bendix Corporation | business | — | 1924 |
| Bennington College | college, private not-for-profit educational institution | Bennington | 1932 |
| Bentley University | private not-for-profit educational institution, university | Waltham | 1917 |
| Berea College | liberal arts college, private not-for-profit educational institution, school | Berea | 1855 |
| Bergdorf Goodman | department store, business | New York City | 1929 |
| Berkeley College | college, private for-profit educational institution | — | 1931 |
| Bethel University | church college, private not-for-profit educational institution, university | — | 1871 |
| Bethlehem Shipbuilding Corporation | shipbuilding company | San Francisco | 1905 |
| Bethlehem Steel | business, enterprise | Bethlehem | 1857 |
| Beverly Hills High School | public school, high school | — | 1927 |
| Beverly Wilshire Hotel | hotel, palace hotel | — | 1928 |
| BFG Technologies | business, enterprise | Lake Forest | 2002 |
| Bicycle Playing Cards | business | Erlanger | — |
| Big Boy Restaurants | restaurant chain, business | Warren | 1936 |
| Big Huge Games | video game developer | Timonium | February 2000 |
| Bigelow Aerospace | startup company, privately held company, business | North Las Vegas | 1999 |
| Billboard | periodical, weekly magazine, publishing house, website | New York City | 1894 |
| Biltmore Hotel | hotel | — | 1926 |
| Biogen | biopharmaceutical company, business, public company | Cambridge | 1978 |
| BioRxiv | document repository, preprint server, disciplinary repository | — | November 2013 |
| BitChute | dot-com company, online video platform | Sheridan, Gravesend | January 2017 |
| Bitly | business, URL shortener | New York City | 2008 |
| Blackboard | business, subsidiary company, public company | Washington, D.C. | 1997 |
| Bloomberg L.P. | business, enterprise | New York City, 731 Lexington Avenue | October 1, 1981 |
| Blu Products | business | Doral, Paramount | 2009 |
| BlueSky Software | video game developer | California | 1988 |
| BlueStacks | business | — | 2011 |
| Blumhouse Productions | film production company | Los Angeles | 2000 |
| Blur Studio | animation studio, business, enterprise | Venice | 1995 |
| BMG Rights Management | military division, record label, music publisher, business | Quartier 205 | October 1, 2008 |
| BNSF Railway | railway company, railway network | Fort Worth | September 22, 1995 |
| Bobcat Company | business | West Fargo | 1947 |
| Boeing | rail vehicle manufacturer, public company, aerospace manufacturer | Chicago | 1934 |